TCNJ Chemistry Instrumentation

Operating Instructions

NMR Sample Prep

General Notes

1) Before you analyze your sample by NMR, the sample must be checked for homogeneity. Prepare your sample in a test tube and inspect it for homogeneity. If necessary, filter the sample into the NMR tube by forming a Kim Wipe "plug" in the top of the tube, and then pipetting your sample into the tube through the plug. (The Kim Wipe acts as a filter.)

2) Use high resolution tubes to analyze your samples on both the 60MHz and 300MHz instruments.

3) Label your sample tube for short term storage using the white hang tags. Do not use tape! The tape residue will damage the probe inside the instrument.

4) Do not leave sample tubes in the sample racks near the instruments for an extended period of time (> 1 week). The tubes will be ruined if chemicals remain in them for an extended period of time!

5) The sample racks in the NMR room will be monitored. Sample tubes in these racks will be removed after ~ 1 week and quarantined.

6) Clean your tubes when you are finished with them. The tubes will be reused.

7) Please take care not to break or damage the sample tubes. They are very expensive to replace (>$10/tube)!

Use the Following Guide When Preparing NMR Samples: