tcnj logo



Growing OP50 to Seed NGM Plates


  • Disposable Pipette Tips
  • L-Broth
  • Snap-Top Plastic Test Tube (i.e. Falcon, 352051)
  • Stock OP50 Plate


  1. Transfer 3-5 mls of L-Broth to the test tube(s). Make sure to do this using sterile technique. Flame the lid and top of the original L-Broth when you open it and when you close it to maintain sterility.
  2. Pick a small amount of bacteria using a pipette tip off the original plate and transfer it to a tube of L-Broth. (Figure 1) This is easier if you have the tip on a micro-piptettor, rather than just the tip alone. (Figure 2)
  3. Let the test tube(s) grow overnight in an incubator/shaker at 37°C.


Figure 1.

Pick the bacteria using a micropipette.

back to procedure


Figure 2.

The micropipette makes it easier to put the bacteria down inside the tube.

back to procedure


Sudhir Nayak, Ph.D.

Biology Building 126
2000 Pennington Rd.
Ewing, NJ 08628
P) 609-771-2659


Dr. Nayak’s Lab
Biology Building 236
P) 609-771-3436