Growing OP50 to Seed NGM Plates
- Disposable Pipette Tips
- Snap-Top Plastic Test Tube (i.e. Falcon, 352051)
- Stock OP50 Plate
- Transfer 3-5 mls of L-Broth to the test tube(s). Make sure to do this using sterile technique. Flame the lid and top of the original L-Broth when you open it and when you close it to maintain sterility.
- Pick a small amount of bacteria using a pipette tip off the original plate and transfer it to a tube of L-Broth. (Figure 1) This is easier if you have the tip on a micro-piptettor, rather than just the tip alone. (Figure 2)
- Let the test tube(s) grow overnight in an incubator/shaker at 37°C.
Pick the bacteria using a micropipette.
The micropipette makes it easier to put the bacteria down inside the tube.