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Streaking OP50

NOTE: OP50 is the bacteria used to seed NGM Plates.


  • Disposable Pipet Tips
  • Gloves
  • LB Plates
  • Stock Plate of OP50


  1. Wear gloves while performing this procedure.
  2. Pick a small amount of OP50 from an old, stock plate of the bacteria using a pipet tip. (Figure 1)
  3. Spread the bacteria back and forth onto a small area of new plate. Make sure that you use a light touch so you don't slice the agar. (Figure 2)
  4. Use a new pipet tip and streak the newly- added bacteria outwards from an area near the end of the initial streak.
  5. Repeat the process one more time using a new pipet tip. See the figure below for the final streaking pattern. (Figure 3)
  6. Grow overnight at 37°C.
  7. Remove from incubator in the morning and store the new OP50 plate(s) at 4°C. They will be good for a month or two, or if they become contaminated.


Figure 1.

Pick the bacteria off of a stock plate using a disposable pipet tip.

back to procedure


Figure 2.

Spread the bacteria on the new LB Plate using a light touch.

back to procedure


Figure 3.

Streaking patern of the new OP50 plate.

back to procedure


Sudhir Nayak, Ph.D.

Biology Building 126
2000 Pennington Rd.
Ewing, NJ 08628
P) 609-771-2659


Dr. Nayak’s Lab
Biology Building 236
P) 609-771-3436