Determining Genetic Variation of White-tailed Deer Populations in New Jersey throughout Time

Krysta Johns-Harris,  Biology

Faculty Mentor: Dr. James Bricker

              The population of White-Tailed Deer in New Jersey was close to extinction around 1900 due to excessive hunting. To replenish the deer population, deer were brought from Pennsylvania and released in New Jersey. Since then, the deer population has increased greatly.  The purpose of this study is to determine the genetic variability between deer from before 1900, during the 1960’s and presently in New Jersey.  We are determining genetic variability between populations using microsatellite loci. Microsatellites are segments of DNA with repeated sequences. Over a period of time, a population will develop a variety of characteristic microsatellites that are specific for that are distinct from other populations.

              This summer my research partner and I successfully extracted DNA from 69 present day White-Tailed Deer samples. All samples were amplified using Polymerase Chain Reaction (PCR) and then agarose gel electrophoresis was used to determine the size of the amplified DNA from each sample.  Each sample had a PCR product of around 160bp.  We are in the process of using a DNA sequencer to discover the sequence of the PCR products.  These results will be compared to samples from before the 1900’s and during the 1960’s.  Deer skulls and antlers from before the 1900’s and during the 1960’s will be obtained from museums.

Personal Statement

              I feel extremely fortunate to be a part of the Summer Undergraduate Research Program (SURP).  Being involved in research taught me things that I cannot learn from a textbook.  I have become skillful in extracting DNA from animal tissue, Polymerase Chain Reaction, and gel electrophoresis.  Not only did I learn procedures, but I also learned life lessons.  I have become more meticulous, patient and determined.  I have had to troubleshoot many times while working in lab, and I have gained so much confidence in my abilities to solve problems.  SURP was my first research experience and I felt extremely comfortable in the supportive lab environment created by my research professor.  One of my favorite parts of this research experience was the lunch discussions.  I was able to learn about other research projects in different subjects that my peers felt passionate about.  I feel the lunch discussions also provided students across different departments to socialize and build relationships with all the students who participated in SURP.  Overall, I feel as if SURP was a very positive experience.  I have learned many things throughout this summer that I am sure will benefit me not only in my future academic goals, but also in life.