Protocols

This page contains commonly used protocols and reagents that my undergraduate lab team have adapted and modified into highly detailed step-by-step instructions for use by novice C. elegans researchers.  Feel free and use our protocols and contact us to let us know how we can optimize them.

Plate Preparation

• Nematode Growth Media (NGM) Plates
• Luria-Bertani (LB) Plates
• LB-Antibiotic Plates
• RNA Interference (RNAi) Plates with IPTG/Lactose
• Preparation and Seeding of NGM Plates
  • Streaking New OP50 Plate
  • Growing OP50 to Seed NGM Plates
• Preparation and Seeding of RNAi Plates
  • Streaking RNAi Bacteria
  • Growing RNAi Bacteria

General Techniques

• 2.0% Agar Pads
• Antibody Staining
• DAPI Staining
• Dissecting Worm
• Drawn Pasteur Pipette
• Freezing Worms
• Gonad Fixation
• L-Broth
• Quick Water Immersion Cooling (QWIC)